Dr David Ryan


Director of the Office of Institute Planning and Research

Office of Planning and Research, Carlow Campus

e: david.ryan@itcarlow.ie      t: 059 9175003

ORCID ID:0000-0002-9655-2373

Dr. David Ryan is a graduate of the National University of Ireland in Galway where he completed his undergraduate studies in biotechnology in the department of biochemistry and his PhD in the environmental research unit at NUIG, under the supervision of Professor Emer Colleran.  He has been based at the Institute of Technology in Carlow since 2000, initially as a postdoc in EnviroCORE before taking on the role of lecturer in department of Science and Health.  He was Head of the Department from 2003-2015 before taking on the role of Assistant Registrar and he is currently the Director of the Office of Institute Planning and Research.


Academic and Research Experience

Dr. David Ryan has lectured at undergraduate and postgraduate in Microbiology, Biochemistry, Bioinformatics, Communication, Innovation, Research Methods, First Year Experience and has supervised final year research dissertations.  He has been involved in programme development at level 8 and 9 in both the department of science and health and in life-long learning and is currently the programme director for the honours degree in Brewing and Distilling at the Institute of Technology in Carlow.  Dr. Ryan’s research interests include microbial metal resistance systems, soil health and bioremediation, the use of endophytic and rhizospheric microbial inoculants for plant growth promotion, both biofuel and cash crops including barley for the brewing and distilling industry.  He has also worked on the development of chemical and microbial based biosensors to detect both contamination and degradation of target compounds, phytotechnologies for biofuel and bioenergy crops, strategies for the acceleration and assessment of the decomposition of biodegradable municipal waste and run off, plant transformation systems, enhancing anaerobic digestion systems.

He has supervised Masters and PhD students, acted as external examiner for levels 8, 9 and 10 and been involved in new programme panels, strategic, programmatic and professional support quality reviews nationally and internationally.  Dr. Ryan is based at the EnviroCORE research unit at the Institute of Technology in Carlow His research has resulted in the formation of a spin out campus-company, MicroGen biotech (www.microgenbiotech.com), with a focus on soil health, plant growth promotion and food safety.


Publications and Outputs

Peer Reviewed Journal Articles

Bhatnagar, N., Ryan, D., Murphy, R. and Enright, A.M. Effect of Co-digestion Ratio and Enzyme Treatment on Biogas Production from Grass Silage and Chicken Litter. (2018) Waste and Biomass Valorization. DOI 10.1007/s12649-018-0377-8

Finnegan, C., Garcia-Cabellos, G., Enright, AM and Ryan, D. ‘Gas Chromatographic Approach to Evaluate the Efficacy of Organotin Degrading Microbes’ (2017) International Journal of Bioremediation and Biodegradation.

Moreira, AS. Germaine, KJ, Lloyd, A, Lally, RD, Galbally, PT, Ryan, D, Dowling, DN  (2016) Draft Genome Sequence of Three Endophyte Strains of Pseudomonas fluorescens Isolated from Miscanthus giganteus Genome Announcements 4(5):965-981 · DOI: 10.1128/genomeA.00965-16 

Otieno, N., Lally, R., Kiwanuka, S., Lloyd, A., Germaine, K., Ryan, D., Germaine, K., and Dowling D.N. (2015) ‘Plant growth promotion induced by phosphate solubilising endophytic Pseuddomonas isoltes’ Frontiers in Microbiology 6:745:1-8

Xuemei Liu, X., Kiwanuka, S., Ryan, D., Dowling D., and Germaine, K. ‘Use of Ecopiling in a Field Trial to Remediate Heavily Impacted Creosote Contaminated Sediment and Soil. Journal of Environmental pollution in January 2015

Germaine, K., Byrne, J., Liu, X., Keohane, J., Culhane, J., Lally, R., Kiwanuka, S., Ryan, D. and Dowling, D.N. (2015) ‘Ecopiling: a combined phytoremediation and passive biopiling system for remediating hydrocarbon impacted soils at field scale’ Frontiers in plant science 5 (756) 1-6

Lally, R., Moreira, A.S., Galbally, P., Ryan, D., Germaine, K., and Dowling, D.N (2013) Application of Endophytic Bacteria Possessing Plant Growth Promoting Traits for the Use in Bioenergy Crop Brassica napus  Aspects of Applied Biology 120: 9-13

Otieno, N., Culhane, J., Germaine, K., Brazil, D., Ryan, D., and Dowling D.N. (2013) Screening of large collections of plant associated bacteria for effective plant growth promotion and colonisation.  Aspects of Applied Biology 120: 23-28

Power, B, Liu, X., Germaine, K., Ryan, D., Brazil D and D. N. Dowling (2011). Alginate beads as a storage, delivery and containment system for genetically modified PCB degrader and PCB biosensor derivatives of Pseudomonas fluorescens F113. Journal of Applied Microbiology. 110(5):1351-8.

Liu X, Germaine KJ, Ryan D and Dowling D (2010). Genetically modified Pseudomonas biosensing biodegraders to detect PCB and chlorobenzoate bioavailability and biodegradation in contaminated soils. Bioengineered Bugs 1:3, 1-9.

Liu, X., Germaine, K.J., Ryan, D. and Dowling, D.N. (2010).  Whole cell fluorescent biosensors for bioavailability and biodegradation of polychlorinated biphenyls. Sensors 10: 1377-1398

Germaine K.J., Keogh E., Ryan, D. and Dowling, D.N. (2009). Bacterial endophyte-mediated naphthalene phytoprotection and phytoremediation. FEMS Microbiol. Lett. 2009, 296, 226-234.

Ryan, R.P., Germaine, K., Franks, A., Ryan, D.J. and Dowling, D.N. (2008).  Bacterial endophytes: recent developments and applications. FEMS Microbiol. Lett.  278:1-9

Ryan, R. P., Ryan, D. and Dowling, D. (2007).  Plant protection by the recombinant, root-colonising Pseudomonas fluorescens F113rifPCB strain expressing arsenic resistance: improving rhizoremediation Letters in Applied Microbiology 45: 668-674

Ryan, R. P, Ryan, D., Sun, YC., Li, FM., Wang, Y. and Dowling, D. (2007).  An acquired efflux system is responsible for copper resistance in Xanthomonas strain IG-8 isolated from China FEMS Microbiol Lett. 268: 40-6.

Liu, X., Germaine, KJ., Ryan, D. and Dowling, DN .(2007)  Development of a GFP-based biosensor for detecting the bioavailability and biodegradation of polychlorinated biphenyls (PCBs). (JEELM) Journal of Environmental Engineering and Landscape Management 15:261-268.

Germaine, K.J, Liu, X., Garcia-Cabellos, G., Hogan, J.P., Ryan, D. and Dowling, D.N. (2006).   Bacterial endophyte- enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid. FEMS Microbiology Ecology 57: 302-310.

Gilmartin, N., Ryan, D. and Dowling, D.N (2005).  Analysis of the C-terminal domain of Burkholderia sp. strain LB400 BphK reveals a conserved motif that affects catalytic activity. FEMS Microbiology Letters 249: 23-30.

Ryan, R.P., Ryan, D. and Dowling, D.N. (2005).   Multiple metal resistant transferable phenotypes in bacteria as indicators of soil contamination with heavy metals. Journal of Soils & Sediments 5:95-100.

Germaine, K, Garcia-Cabellos, G., Borremans B., van der Lelie, N., Barak, T., Oeyen, L., Vangronsveld, J., Porteous-Moore, F., Moore, E., Campbell, C., Ryan, D. and Dowling D. (2004).  Colonisation of Poplar trees by gfp expressing bacterial endophytes FEMS Microbial Ecology 48: 109-118

Hogan, J; Sherlock, O; Ryan, D; Whelan, C; Francesconi, S., Rivilla, R. and Dowling, D.  (2004).  Fluorescence resonance energy transfer (FRET) based molecular detection of a genetically modified PCB degrader in soil.  FEMS Microbiology Letters 236:349-357

Ryan R. P, Ryan D., Dowling D. (2004).  “The isolation and characterisation of a multiple heavy metal resistant plasmid pITCRR46 from a heavy metal contaminated site” In: The BioNet Conference ISSN publication.

Gilmartin, Niamh., Ryan, D., Sherlock, O., and Dowling, D., (2003).  A potential role for the bphk gene encoding a Glutathione S-transferase in the PCB breakdown pathway” FEMS Microbial Letters.

Gilmartin, N., Ryan, D., Sherlock, O. and Dowling, D. (2003).  BphK shows dechlorination activity against 4-chlorobenzoate, an end product of bph-promoted degradation of PCBs. FEMS Microbiology Letters 222:251-255.

Ryan, D. and Colleran, E. Arsenical resistance in the IncHI2 plasmids (2002).  Plasmid, 47, 234-240

Books and Book Chapters

Ryan R.P, Ryan, D. and Dowling D. (2008). Heavy metal tolerant rhizobacteria and their phytoremediation potential. In: Phytoremediation and Rhizoremediation: Focus on Biotechnology. Vol 9B. Edited by Mackova M, Dowling DN, Macek T. Springer.

Garcia-Cabellos, G., Byrne, M., Stenberg, M., Germaine, K., Brazil, D., Keohane, J., Ryan, D. and Dowling, D. (2005) A small scale constructed wetland to treat different types of wastewaters. In: Nutrient Management in Agricultural Watersheds: A Wetlands Solution, pp224-229 edited by E. J. Dunne, K.R., Reddy and O.T. Carton. Wageningen Academic Publishers, The Netherlands.

Conference Presentations

Approximately 100 to date

Other Research Outputs

Finnegan, C., Enright, AM., Ryan, D and Garcia-Cabellos, G.  'Bioremediation of Tributyltin (TBT) in Irish marine sediments: Microbial screening and process optimisation'. Presented at Environ 2017 – winner of the ESAI Postgraduate of the Year Award.

Richard D. Lally, David Ryan, Andrew Lloyd, Kieran J. Germaine and David N. Dowling ‘Plant growth promoting Pseudomonads, their colonisation dynamics and agricultural potential’ Winner of the Alltech Young Scientist program 2016.

Research Supervision

Applied research projects primarily in microbial plant growth promotion, soil bioremediation, alternative energy and/or heavy metal analysis and the development of commercial and social IP is important in all of my research.  I am planning to expand my focus to include the microbial microbiome and its potential for influencing crops used in the brewing and distilling industries.

Current Research Students

Name of Student

Title of Thesis



Cormac Griffin

The application of microbial consortium constructed by functional microbiome technology for preventing absorption of cadmium by crop plants



Wojciech Nowak

Plant growth promotion potential of novel bacterium Ensifer adhaerens OV14



Navoditar Bhatnagar

Influence of enzyme supplementation technologies on biogas production and productivity in agriculture



Evelyn Joyce

In-situ bioremediation and molecular microbiological monitoring of ammonia contamination in shallow groundwater



Christopher Finnegan

Bioremediation of Tributyltin (TBT) in Irish marine sediments: Microbial screening and process optimisation



Annija Lace

‘Optochemical detection strategies for heavy metals in water’



Sinead Morris

Microbial bioremediation and physiochemical treatment of landfill leachate



Aisling Dunne

Microbial interactions of the novel bacterium Ensifer adhaerens OV14 with economically important crops



Past Research Students

Name of Student

Title of Thesis



Robert Ryan

The generation and molecular analysis of multi-resistant microbial strains and associated operons for the bioremediation of toxic metals



Jill Hogan

The monitoring of a prokaryotic bioremediation system in PCB contaminated soil and their in-situ quantification



Kieran Germaine

Construction of endophytic xenobiotic degrader bacteria for improving the phytoremediation of organic pollutants



Xuemei Liu

Development and application of biosensor technologies for the biodegradation of environmental pollutants



Elaine Keogh

The isolation and characterisation of bacterial endophytes and their potential applications for improving phytoremediation



Monica Dempsey

Evaluating microbial biosensors to detect polychlorinated biphenyl (PCB) contamination and degradation in a range of environments and conditions



Olivia Odhiambo

Developing improved phytoremediation and phytoprotection systems



Karen Browne

Characterisation of tributyltin resistant microbes isolated from Irish coastal regions



Nicholas Otieno

Development of endophytic bacterial inoculants possessing plant growth promotion traits for practical application in bioenergy plant species



Richard Lally

Examination of plant growth promoting bacteria on plant yield and their effect as bio-insecticides.



Leo Fleming Farrell

A Study of European Medical Device Regulations Effectiveness in the Prevention of Product Recalls 




Engagement and Collaboration

All of my research projects are applied in nature and I work with a number of companies and organisations including Alltech, TelLabs, Teagasc, MicroGen Biotech, and WIT.  I have also worked with a number of breweries and distilleries on product development innovation and analysis projects.