Dr Thomae Kakouli-Duarte

Research Interests

I have research experience in the areas of agri-environmental science, specifically, biological control of insect pests using entomopathogenic nematodes, the development of DNA-based diagnostics of quarantine insect pests, bumblebee molecular ecology and conservation, and the application of nematodes and their assemblages as indicators or environmental change.  I have commenced my career at undergraduate level obtaining a BSc in Technology of Crop Production from the Technological Educational Institute of Western Macedonia, Greece, and then I qualified at MSc level in the Technology of Crop Protection at the University of Reading, UK. In Reading, I also obtained a PhD on the biological control of Otiorhunchs sulcatus using entomopathogenic nematodes. I then completed two postdoctoral fellowships in Maynooth University, on the molecular genetics for entomopathogenic nematodes and on the development of molecular probes for the identification of tephritid fruit flies. Subsequent to these, I was appointed Assistant Lecturer at SETU Carlow, and I am currently a Lecturer in the Department of Science Health. I am also the Director of enviroCORE in SETU Carlow. My research interests encompass the areas that I have mentioned above, and have expanded in the areas of sustainable plant parasitic nematode management and vermitechnology.

Publications

Peer Reviewed Journal Articles

Moreira, A.S.C.F, Horgan, F., Murray, T., Kakouli-Duarte, T. 2015. Population genetic structure of Bombus terrestris in Europe: isolation and genetic differentiation of Irish and British populations. Molecular Ecology 24 (13): 3257-3268. DOI: 10.1111/mec.13235

Moreira, A.S.C.F, Horgan, F., Murray, T., Kakouli-Duarte, T. 2013.  Bumblebee (Hymenoptera: Apidae) sample storage for a posteriori molecular studies: interactions between sample storage and DNA-extraction techniques. European Journal of Entomology 110(3): 419-425. DOI: 10.14411/eje.2013.056.

Rolston, A., Meade, C., Boyle, S., Kakouli-Duarte, T. and Downes, M. 2009. Intraspecific variation among isolates of the entomopathogenic nematode Steinernema feltiae from Bull Island, Ireland. Nematology, 11(3): 439-451.

Athanassiou, C.G., Palyvos, N.E. and Kakouli-Duarte, T. 2008. Insecticidal effect of Steinernema feltiae (Filipjev) (Nematoda: Steinernematidae) against Tribolium confusum du Val (Coleoptera: Tenebrionidae) and Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae) in stored wheat. Journal of Stored Products Research 44: 52-57.         

Boyle, S. and Kakouli-Duarte, T. 2008. The effects of chromium VI on the fitness and on the β-tubulin genes during in vivo development of the nematode Steinernema feltiae. Science of the Total Environment, 404: 56-67.

Valadas, V., Boyle, S., Vieira, P., Kakouli-Duarte, T. and Mota, M. 2007. First report on entomopathogenic nematodes from continental Portugal. Helminthologia, 44: 226-229.

Rolston, A.N., S. Boyle, T. Kakouli-Duarte, C.T .Griffin and M.J. Downes. 2004. Distribution of, and intraspecific variation between, populations of entomopathogenic nematodes from Bull Island, Republic of Ireland.  Journal of Nematology 36 (3): 344.

Kakouli-Duarte T., Casey D.G., and Burnell, A.M. 2001. Development of a diagnostic DNA probe for the fruit flies Ceratitis capitata and Ceratitis rosa (Diptera: Tephritidae) using AFLP. Journal of Economic Entomology. 94: 989-997.

Kakouli-Duarte T. and Hague, N.G.M. 1998. Infection, development, and reproduction of the entomopathogenic nematode Steinernema arenarium in the black vine weevil Otiorhynchus sulcatus.  Nematology 1: 149-156.

Kakouli-Duarte T., Labuschagne, L. and Hague, N.G.M. 1997. Biological control of the black vine weevil, Otiorhynchus sulcatus (Coleoptera: Curculionidae) with entomopathogenic nematodes (Nematoda: Rhabditidae). Annals of applied Biology, 131: 11-27.

Submitted

Boyle, S., Kakouli-Duarte, T. 2018. The behaviour of the nematode Steinernema feltiae (Nematoda: Steinernematidae) in sand contaminated with the industrial pollutant, chromium VI. Ecotoxicology.

Manuscripts in Preparation

Boyle, S., Mestre Alvarez, L. and Kakouli-Duarte, T. 2018. The behaviour of the nematode, Steinernema feltiae (Nematoda: Steinernematidae) in sand contaminated with the industrial pollutant, chromium VI. Ecotoxicology.

Boyle, S. and Kakouli-Duarte, T. 2018. The β-tubulin genes in the nematode Steinernema feltiae. Journal of Nematology.

Nguyen, V.T. and Kakouli-Duarte, T. 2018. Evaluation of nematode community structure as an indicator of terrestrial heavy metal contamination; site specific effects on an Irish mining site. Environmental Toxicology and Chemistry. 

Kelly, E. and Kakouli-Duarte, T. 2018. Evaluation of Steinernema feltiae as a bioindicator of heavy metal contamination using toxicity assays. Ecotoxicology and Environmental Safety.

Kelly, E. and Kakouli-Duarte, T. 2018. Effects of heavy metals on the biology the nematode Steinernema feltiae. Nematology.

Books and Book Chapters

M. J. Wilson and T. Kakouli-Duarte. 2009. Nematodes as Environmental Indicators. CABI, Wallingford, UK.  DOI 10.1079/9781845933852.0000

Conference Proceedings and Papers

Invited and Keynote Talks

Kakouli-Duarte, T. 2016. Nematology and environmental change: perspectives, challenges and the future. Keynote Opening Address on Session “Nematodes as bio-indicators in terrestrial, freshwater and marine habitats”. 32^nd Symposium of the European Society of Nematologists. University of Minho, Braga, Portugal, 28 August – 1 September 2016.

Kakouli-Duarte, T. 2013. Humans, C. elegans and DNA: a worm’s tale. Keynote Address in the Regional European Biomedical Laboratory Science Congress and the 4^th Greek Medical Laboratory Technologists Conference. Athens, Greece, 5-7 December 2013.

Kakouli-Duarte, T. 2009. Invited: Soil nematode diversity and its function as an environmental indicator. Soil Biodiversity Research in Ireland and Britain, Satellite Workshop to the BSSS-SSSI Joint Conference, Teagasc, Johnstown Castle, Wexford, Ireland, 8 September 2009.

Kakouli-Duarte, T. and A. M. Burnell. 1998. Invited: The detection and isolation of interspecific genetic markers in tephritid flies using Amplified Fragment Length Polymorhisms (AFLPs). Invited by Prof. C. Savakis, Foundation for Research and Technology, Institute of Molecular Biology and Biotechnology, University of Crete, Greece. University of Crete, 21 August 1998.eraklionH

Kakouli-Duarte, T. 1996. Invited: Biological control of the black vine weevil, Otiorhynchus sulcatus (F.) (Coleoptera: Curculionidae), with entomopathogenic nematodes (Nematoda: Rhabditida). Invited by Professor R. J. Marks, Deputy Chief Officer, Applied Plant Science Division, Dept. of Agriculture for N.I., and Head of Department, Dept. of Applied Plant Science, The Queen's University of Belfast. The Queen's University of Belfast, Northern Ireland, 14 May 1996.

Kakouli-Duarte, T., K. Hanlon and A. M. Burnell. 1996. Invited: The identification and characterisation of active transposable elements in the entomopathogenic nematodes Heterorhabditis spp. (Nematoda: Rhabditida). Invited by Dr D. Finnegan, Director of the Institute of Cell and Molecular Biology, University of Edinburgh. University of Edinburgh, 3 October 1996.

Conference Abstracts and Proceedings

Egan, A., Kakouli-Duarte, T. 2017. Classical and novel approaches in using nematodes as indicators of endophytic bacterial bioremediation in an oilseed rape crop. 3rd International Symposium on Nematodes as Environmental Bioindicators. The Association of Applied Biologists. Institute of Technology Carlow, Ireland. 28 – 29 June 2017.

Fitzpatrick, M., Lloyd, A., Kakouli-Duarte, T. 2017. Preliminary investigations on the utilisation of vermitea as an alternative nutrient source for plan growth. Environ 2017. ESAI. Athlone Institute of Technology. 10-12 April 2017.

Kakouli-Duarte, T. 2017. Nematodes and environmental change. Environ 2017. ESAI. Athlone Institute of Technology. 10-12 April 2017.

Mwana-Scoe, L., Germaine, K., Kakouli-Duarte, T., Cleary, J. 2017. Assessment of microplastics using nematodes as environmental indicators. Environ 2017. ESAI. Athlone Institute of Technology. 10-12 April 2017.

Mwana-Scoe, L., Germaine, K., Kakouli-Duarte, T., Cleary, J. 2017. A preliminary investigation on the effects of contaminated microplastics using nematodes as environmental indicators. 3^rd International Symposium on Nematodes as Environmental Bioindicators. The Association of Applied Biologists. Institute of Technology Carlow, Ireland. 28 – 29 June 2017.

Mwana-Scoe, L., Germaine, K., Kakouli-Duarte, T., Cleary, J. 2017. Characterisation of microplastics in Irish freshwaters. CEST 2017: The 15^th International Conference on Environmental Science and Technology, Rhodes, Greece. 31 August-2 September 2017.

Uhlemann, J., Kakouli-Duarte, T, Cawley, O. 2017. Computer assisted identification, counting, sorting and monitoring in biological systems. 3rd International Symposium on Nematodes as Environmental Bioindicators. The Association of Applied Biologists. Institute of Technology Carlow, Ireland. 28 – 29 June 2017.

Egan, A., Kakouli-Duarte, T. 2016. Evaluation of the bioremediating ability of bacterial endophytes using nematodes as bioindicators in a bioenergy crop. 32^nd Symposium of the European Society of Nematologists. University of Minho, Braga, Portugal, 28 August – 1 September 2016.

Egan, A., Kakouli-Duarte, T. 2016. An investigation into plant-parasitic nematode and endophytic bacterial interactions. 32^nd Symposium of the European Society of Nematologists. University of Minho, Braga, Portugal, 28 August – 1 September 2016.

Hurley, M.J., Brazil, D., Kakouli-Duarte, T., 2016. The effects of endophytic bacteria on entomopathogenic virulence and infectivity. 32^nd Symposium of the European Society of Nematologists. University of Minho, Braga, Portugal, 28 August – 1 September 2016.

Kakouli-Duarte, T., Kelly, E. 2016. The receiver operating characteristic curve application in environmental nematology. 32^nd Symposium of the European Society of Nematologists. University of Minho, Braga, Portugal, 28 August – 1 September 2016.

Fitzpatrick, M., Lloyd, A., Kakouli-Duarte, T. 2016. Investigation of Vermitechnology: A green waste management method for food waste. 26^th Irish Environmental Researchers’ Colloquium, ENVIRON 2016. University of Limerick, 22-24 March 2016.

Moreira, A.S., Horgan, F.G., Murray, T.E., Kakouli-Duarte, T. 2015. Population genetic structure of Bombus terrestris in Europe: Conservation implications in Ireland and Britain. 16^th Panhellenic Entomological Congress. Heraklion, Greece, 20-23 October 2015.

Hurley, M.J, Brazil, D., Kakouli-Duarte, T. 2015. Entomopathogenic nematode and endophytic bacteria interactions. Rhizosphere 4. Maastricht, The Netherlands, 21-25 June 2015.

Egan, A., Kakouli-Duarte, T. 2015. Evaluation of the bioremediating ability of bacterial endophytes using nematodes as bioindicators in a bioenergy crop. Rhizosphere 4. Maastricht, The Netherlands, 21-25 June 2015.

Hurley, M.J., Kakouli-Duarte, T. 2014. Entomopathogenic nematodes and endophytic bacteria: a novel approach with potential for plant health. 6^th International Congress of Nematology. Cape Town, South Africa, 4-9 May 2014.

Kelly, E., Kakouli-Duarte, T. 2014. Investigating Steinernema feltiae as a suitable indicator organism for heavy metal toxicity. 6^th International Congress of Nematology. Cape Town, South Africa, 4-9 May 2014.

Moran, S., Kakouli-Duarte, T. 2013. Searching for markers of local climate adaptation in the genomes of Bombus terrestris populations originating from a variety of habitats. Environ2013, ESAI, NUIG, 30 January – 1 February 2013.

Moran, S., Kakouli-Duarte, T. 2013. Development of geographic markers for European Bombus terrestris using the RAPD approach. Environ2013, ESAI, NUIG, 30 January – 1 February 2013.

Egan, A., Kakouli-Duarte, T. 2012. Use of nematodes to evaluate the bioremediating ability of bacterial endophytes in bioenergy crops. 2^nd International Symposium on Nematodes as Environmental Bioindicators, The Association of Applied Biologists, University of Ghent, Ghent, Belgium, 6-7 July 2012.

Hurley, M. J., Kakouli-Duarte, T. 2012. Effects of bacterial endophytes on the survival and virulence of entomopathogenic nematodes. Advances in Nematology. The Association of Applied Biologists. The Linnean Society of London, Piccadilly, London, UK. 11 December 2012.

Nguyen, V.T., Kakouli-Duarte, T. 2012. Analysis of the 28S rRNA gene in nematodes under heavy metal stress. 2^nd International Symposium on Nematodes as Environmental Bioindicators, The Association of Applied Biologists, University of Ghent, Ghent, Belgium, 6-7 July 2012.

Nguyen, V. T., Kakouli-Duarte, T. 2011. Temporal changes in soil nematode community structure under heavy metal stress in an Irish mining site. 12^th European Ecological Federation Congress. Ávila, Spain, 25-29 September 2011.

Desta, T. A., Kakouli-Duarte, T. 2010. Biodiversity and vertical distribution of nematodes from the rhizosphere of bioenergy ctrops. Proceedings of the Fourth Annual Meeting of COST Action 872: Nemagenics – Exploiting genomics to understand plant-nematode interactions. Lisbon, Portugal, 24-27 May 2010.

Nguyen, V. T., Kakouli-Duarte, T. 2010. Analysis of nematode communities in an Irish mining area highly contaminated with heavy metals. 30^th International Symposium of the European Society of Nematologists. Vienna, September 19-23 2010.

Nguyen, V.T., Kakouli-Duarte, T. 2010. Assessment of nematode genetic diversity with mtDNA and its function as an ecotoxicological tool in heavy metal contaminated soils. Proceedings of the Fourth Annual Meeting of COST Action 872: Nemagenics – Exploiting genomics to understand plant-nematode interactions. Lisbon, Portugal, 24-27 May 2010.

Nguyen, V.T., Kakouli-Duarte, T. 2010. Nematodes as a bioindicator for heavy metal soil contamination in Ireland. Environmental Protection Agency National Research Conference 2010 – “Science into Action for Sustainable Ireland”, Croke Park Conference Centre, Dublin, Ireland. 23 June 2010.

Nguyen, V.T., Kakouli-Duarte, T. 2009. The use of DNA barcoding in nematode ecotoxicology: from species identification to genetic diversity and stability of populations under stress of heavy metal contamination. Proceedings of the Third Annual Meeting of COST Action 872: Nemagenics – Exploiting genomics to understand plant-nematode interactions. Toledo, Spain, 25-28 May 2009. Pp. 58-59

Nguyen, V.T., Kelly, E. Kakouli-Duarte, T. 2009. Community structure and sentinels: observing the effects of heavy metals on nematodes. Advances in Nematology. The Association of Applied Biologists. The Linnean Society of London, UK. 15 December 2009.

Boyle, S., Kakouli-Duarte, T. 2008. A subtracted cDNA library of putative chromium influence genes: a nematode generated resource in ecotoxicology. Proceedings of the 5^th International Congress of Nematology, 13-18 July, Brisbane, Australia, p. 91.

Kakouli-Duarte, T., Boyle, S., O’Connor, W., Kelly, E, Nguyen, V.T. 2008. Nemagenics for the environment. Proceedings of the second Annual Meeting of COST Action 872: Nemagenics – Exploiting genomics to understand plant-nematode interactions. 26^th -29^th May, 2008, Postojna, Slovenia, p. 62.

Kelly, E., Kakouli-Duarte, T. 2008. Effcets of cadmium, lead and zinc on entomopathogenic nematode Steinernema feltiae.  Proceedings of the 5^th International Congress of Nematology, 13-18 July, Brisbane, Australia, p. 267.

Kelly, E and Kakouli Duarte, T. 2008.  “Observations on the insecticidal ability and of Steinernema feltiae in response to heavy metal exposure”. Advances in Nematology. The Association of Applied Biologists. The Linnean Society of London, UK. 16 December 2008.

Kelly, E, Kakouli-Duarte, T. 2008. The effects of heavy metals on the insecticidal ability of Steinernema feltiae. Environ2008, ESAI, Dundalk Institute of Technology, 1-3 February 2008.

Moreira, S, Horgan, F. Kakouli-Duarte, T. 2008. Irish pollinators: The plight of the bumblebee.  Environ2008, ESAI, Dundalk Institute of Technology, 1-3 February 2008.

Moreira, S, Horgan, F, Murray, T, Kakouli-Duarte, T. 2008. Phylogenetics as Risk Assessment – The case of Bombus terrestris, 9^th Student Conservation Conference, Cambridge University, 25-27 March 2008.

Nguyen, V.T., Moens, T., Kakouli-Duarte, T. 2008. Heavy metal toxicity assessment using nematodes: from bioassay to community analysis. Environ2008, ESAI, Dundalk Institute of Technology, 1-3 February 2008.

Boyle, S., Kakouli-Duarte T. 2007. The nematode Steinernema feltiae and its genome: can they act as indicators of environmental chromium pollution? . 1^st International Symposium on Nematodes as Environmental Bioindicators.  Association of Applied Biologists. Heriot-Watt University, Edinburgh, UK. 12-13 June 2007.

Boyle, S., Kakouli-Duarte, T. 2007. The effects of chromium VI on the b–tubulin genes in Steinernema feltiae. Advances in Nematology. Association of Applied Biologists. The Linnean Society of London, Piccadilly, London, UK. 11 December 2007.

Kakouli-Duarte, T., Boyle, S., O’Connor, W., Kelly, E. 2007. Metals, mines and nematode genes: an Irish perspective. 1^st International Symposium on Nematodes as Environmental Bioindicators.  Association of Applied Biologists. Heriot-Watt University, Edinburgh, UK. 12-13 June 2007.

Kelly, E., Kakouli-Duarte, T. 2007. Nematode geno- and ecotoxicology for environmental monitoring of lead, cadmium and zinc pollution in Irish soils. . 1^st International Symposium on Nematodes as Environmental Bioindicators.  Association of Applied Biologists. Heriot-Watt University, Edinburgh, UK. 12-13 June 2007.

Kelly, E., Kakouli-Duarte, T. 2007. The effects of heavy metals on the insecticidal ability of Steinernema feltiae. Advances in Nematology. Association of Applied Biologists. The Linnean Society of London, Piccadilly, London, UK. 11 December 2007.

O’ Connor W, Dowling, D., Kakouli-Duarte, T. 2007. Nematode SSU rDNA macroarray: a molecular tool capable of identifying  nematode taxa in mixed species soil samples. . 1^st International Symposium on Nematodes as Environmental Bioindicators.  Association of Applied Biologists. Heriot-Watt University, Edinburgh, UK. 12-13 June 2007.

Boyle, S., Kakouli-Duarte, T. 2007. The effects of the industrial contaminant Chromium VI on the entomopathogenic nematode Steinernema feltiae.  Environ 2007. ESAI. Institute of Technology Carlow, Carlow, Ireland. 26-28 January 2007.

O’ Connor, W., Dowling, D., Kakouli-Duarte, T. 2007. Use of 18S rDNA macroarrays to identify individual soil nematode species.  Environ 2007. ESAI. Institute of Technology Carlow, Carlow, Ireland. 26-28 January 2007.

Boyle, S. and Kakouli-Duarte, T. 2006. Effects of the industrial pollutant CrVI on the nematode Steinernema feltiae. Association of Applied Biologists, Advances in Nematology. The Linnean Society, London, UK. 12 December 2006.

Boyle, S., Downes, M., Dowling, D., Kakouli-Duarte, T. 2006. Separation of isolates of the nematode Steinernema feltiae into two distinct genetic groups, based on molecular and behavioural studies. Proceeding of the 28^th International Symposium of the European Society of Nematologists,  Blagoevgrad, Bulgaria, 5-9 June 2006.

O’Connor, W., Dowling, D.N., Kakouli-Duarte, T. 2006. Nematode 18S rDNA: A diagnostic molecular tool with potential in soil bioindicator studies. Proceeding of the 28^th International Symposium of the European Society of Nematologists,  Blagoevgrad, Bulgaria, 5-9 June 2006.

Courtney, L., Kakouli-Duarte, T, Mullins, E. 2006. Towards the development of Sclerotenia trifoliorum resistant clover. The Biotechnology Showcase, BioNet Annual Conference 2006. Institute of Technology Tallaght-Dublin. 18-19 January 2006.

Boyle, S., Dowling, D., Downes, M., Kakouli-Duarte, T. 2005.  Molecular characterisation of Steinernema feltiae isolates using b–tubulin and rDNA genes. Association of Applied Biologists, Advances in Nematology. The Linnean Society, London, UK. 13 December 2005.

O’Connor, W., Dowling, D., Kakouli-Duarte, T. 2005. Development of oligonucleotide probes for nematode molecular diagnostics using rDNA genes. Association of Applied Biologists, Advances in Nematology. The Linnean Society, London, UK. 13 December 2005.

Boyle, S., D. Dowling, M. Downes, T. Kakouli-Duarte. 2005. Base-pair variation in the beta–tubulin gene of Steinernema feltiae: A new approach to differentiate at the strain/isolate level in this nematode.  10^th European Meeting of IOBC “Insect pathogens and Entomoparasitic Nematodes” In cooperation with COST Action 842 and 850 “Entomophthorales” & “Biocontrol Symbiosis”. Locorotondo, Bari, Italy. 10-15 June 2005.

Boyle,  S., D. Dowling and T. Kakouli-Duarte 2004. DNA variation and the tracking of DNA polymorphisms between different generations of entomopathogenic nematodes. The Biotechnology Showcase, BioNet Annual Conference 2004. Institute of Technology Carlow, Carlow, Ireland. 15 – 16 December 2004.

Courtney, L., T. Kakouli – Duarte and E. Mullins 2004. Quantifying the agricultural implications associated with the co – existence of a disease resistant GM and conventional clover variety. The Biotechnology Showcase, BioNet Annual Conference 2004. Institute of Technology Carlow, Carlow, Ireland. 15 – 16 December 2004.

O’ Connor, W., D. Dowling and T. Kakouli – Duarte 2004. Development of a DNA array for soil nematode community characterization. The Biotechnology Showcase, BioNet Annual Conference 2004. Institute of Technology Carlow, Carlow, Ireland. 15 – 16 December 2004.

Boyle, S. and T., Kakouli – Duarte. (2002). The use of the glycosylase – mediated polymorphism detection for the study of gene flow in entomopathogenic nematodes. Proceedings of Fourth International Congress of Nematology, Tenbel, Canary Islands, 8-13 June 2002, Nematology 4 (2).

Boyle, S., T. Kakouli-Duarte and D. Dowling (2003). The Study of Allelic Variation in Entomopathogenic Nematodes (EPNs) Using DNA Based Technologies. Environ 2003, NUI Galway, Ireland, 8– 10 January 2003.

Boyle, S., T. Kakouli-Duarte and D. Dowling (2002). The Use of the Glycosylase Mediated Polymorphism Detection Technique for the study of Gene Flow in Entomopathogenic Nematodes. ISP/BAVP, Faculty of Veterinary Medicine, UCD, Dublin, Ireland, 6-7 September 2002

Boyle, S., T. Kakouli-Duarte and D. Dowling (2002). The Development of a DNA Based Technique for the Study of Gene Flow in Indigenous Entomopathogenic Nematode Populations in Ireland. Association of Applied Biologists, Offered Papers in Nematology, The Linnean Society of London, London, UK. 17 December 2002.

Kakouli-Duarte, T. and N. G. M. Hague (1996). Site Adaptation of the Entomopathogenic Nematode Steinernema anomali (Nematoda: Rhabditidae) to the Black Vine Weevil, Otiorhynchus sulcatus (Coleoptera: Curculionidae). Presented: Irish Zoologists' Meeting 1996, Trinity College, Dublin, Ireland. 21st-22nd March 1996.

Kakouli-Duarte, T., A. Schirocki and N. G. M. Hague (1996). Factors Affecting the Control of Otiorhynchus sulcatus with Entomopathogenic Nematodes, in Strawberries Grown on Raised Beds. Poster presented at: A. A. B. Conference, Economic Nematology, Entomopathogenic Nematodes and Education: A meeting in recognition of N. G. M. Hague. University of  Reading, UK. 25 April 1996.

Kakouli-Duarte, T. and N. G. M. Hague (1996). Infection, Development, Reproduction and Site Adaptation of the Entomopathogenic Nematode Steinernema anomali in the Black Vine Weevil Otiorhynchus sulcatus. XIIth International Colloquium on Soil Zoology, Soil Organisms and Soil Resource Management,  Abstracts: 124. University College Dublin, Ireland. 21-26 July 1996.

Kakouli-Duarte, T. (1996). Susceptibility Studies and Field Control of the Black Vine Weevil, Otiorhynchus sulcatus (F.) (Coleoptera: Curculionidae) with the Entomopathogenic Nematode Steinernema carpocapsae (Nematoda: Rhabditida).  Irish Society for Parasitology Annual Meeting, University College Galway, Galway, Ireland. 13-14 September 1996.

Kakouli-Duarte, T., K. Hanlon and A. M. Burnell (1996). The identification and characterisation of repetitive DNA families in the genome of entomopathogenic nematodes Heterorhabditis spp. Offered Papers in Nematology, A.A.B.  Conference, The Linean Society of London, London, UK. 18 December 1996.

Kakouli-Duarte, T. and N. G. M. Hague (1995). Infection, development and reproduction of the entomopathogenic Nematode Steinernema anomali in the black vine weevil Otiorhynchus sulcatus. Presented: Offered Papers in Nematology, A.A.B.  Conference, The Linean Society of London, London, UK. 13 December 1995.

Kakouli, T., L. Labuschagne and N.G.M. Hague (1994). Nematode  Introduction  through  T-Tapes. Presented: Wilmot Pertwee Horticulture  Technical  Meeting,  H.R.I.,  East  Malling,  Kent,  UK.  January  1994.

Kakouli, T., L. Labuschagne and N. G. M. Hague  (1994). Application of  the Entomopathogenic Nematode Steinernema carpocapsae (UK strain)  through the T-Tape Irrigation System for the Control of the Black Vine  Weevil (Otiorhynchus sulcatus)  in Strawberries Grown on Raised Beds.   Presented: Vine Weevil Workshop. Organiser: L. Labuschagne.   Rochester,  Kent,  UK.  June  1994.

Kakouli, T., L. Labuschagne and N. G. M. Hague (1994). Application of Steinernema carpocapsae (UK  isolate) through a Drip Irrigation System to  Control the Black Vine Weevil Otiorhynchus sulcatus on Strawberries.  Proceedings of the VIth International Colloquium on Invertebrate Pathology and Microbial Control, and IInd International Conference on Bacillus thuringiensis 2: 18. Montpellier, France, 28 August-2 September  1994.   

Kakouli, T., A. Schirocki and N. G. M. Hague (1994). Factors Affecting  the Control of Otiorhynchus sulcatus with Entomopathogenic Nematodes,  in Strawberries Grown on Raised Beds. Proceedings of Brighton Crop Protection Conference, Pests and Diseases-1994 2: 945-946. Brighton,  UK. 21-24 November  1994.

Kakouli, T. and N. G. M. Hague (1994). Susceptibility of Different Stages of the Black Vine Weevil (Otiorhynchus sulcatus) to Entomopathogenic Nematodes. Presented: Offered Papers in Nematology, A.A.B. Conference,  The Linnean Society of London , London, UK.  December  1994.

Gouge,  D.H.,  A.A.  Otto,  T.  Kakouli and N.G.M.  Hague  (1993)  Low Temperature Infection  of  Galleria  mellonella  by  Steinernema  feltiae  and  Steinernema  carpocapsae  between  6.4°C  and  19.2°C.  Proceedings of Cost 812 Conference, Genetics of Entomopathogenic Nematode-Bacterium Complexes: 225.  St.  Patrick’s College,  Maynooth,  Ireland.   October  1993.

Kakouli, T., L. Labuschagne and N.G.M. Hague (1993). A Novel Method for Applying  Entomopathogenic  Nematodes  for  Black  Vine  Weevil  Control in Strawberries. Presented: Offered Papers in Nematology, A.A.B.  Conference,  Rothamstead  Experimental  Station,  UK.  December  1993.

 

Published Reports

Research Supervision

Current Research Students

• Egan, Aoife. Plant parasitic nematode interactions and nematode diversity in bioenergy crops. Principal Investigator. PhD.
• Hurley, Mary Jo.  An investigation on the interactions between entomopathogenic nematodes and bacterial endophytes isolated from bioenergy crops.  Principal Investigator. PhD.
• Fitzpatrick, Mary. Analysis of the vermicomposting process and potential for plant growth promotion under Irish conditions. Principal Investigator. MSc.
• Lee, Sook Thin. Development and formulation to pre-commercialisation state of an innovative bio-control and plant growth-promoting agent for sustainable agriculture. Principal Investigator. MSc.
• Pulavarty, Anusha. Development of sustainable plant parasitic nematode management approaches. Principal Investigator. MSc.
• Mwana-Scoe, Loriane. Characterisation of microplastics in Irish freshwaters. MSc.
• Uhlemann, Jason. Computer assisted counting, identification, sorting and monitoring in biological systems. MSc.

Past Research Students

• Courtney, Lucy. Towards the development of Sclerotinia trifoliorum resistant white clover. MSc. 2006.
• Boyle, Stephen. An investigation of the intraspecific genetic variation and genetic structure of Irish and exotic Steinernema feltiae (Nematoda: Steinernematidae). PhD. 2007.
• O’Connor, William, J.K. The design and development of a DNA array for soil nematode identification. MSc. 2009.
• Moreira, Antonio Sergio Camelo Ferrao. Potential impact of bumblebee commercialisation on population genetic structure of Bombus terrestris L. and Bombus lucorum L. (Hymenoptera: Apidae). PhD. 2012.
• Kelly, Eileen. Evaluating the suitability of the nematode Steinernema feltiae (Nematoda: Steinernematidae) as an environmental indicator. PhD. 2015.
• Moran, Samuel. Circadian gene and genetic variation analyses in insects, with special emphasis on Bombus terrestris L. (Hymenoptera: Apidae). MSc. 2016.
• Lally, Richard. Investigating the application, plant growth promotion and genetic potential of three endophytic Pseudomonas fluorescens isolates for use in sustainable agriculture. PhD. 2017.

Areas of interest as a supervisor include

• Entomopathogenic nematology
• Nematode ecology
• Plant nematology
• Biological control
• Conservation entomology
• Pollinators
• Soil
• Aquatic and benthic macroinvertebrate biodiversity
• Molecular ecology
• Vermitechnology
• waste management
• quarantine molecular diagnostics
• biosensors
• bioindicators
• biomonitoring
• sustainable food security.

Engagement and Collaboration

I have initiated and developed the international symposia on nematodes as environmental bioindicators, under the auspices of the Association of Applied Biologists. I am also a member of the European Society of Nematologists, the Royal Entomological Society Recently, the Environmental Sciences Association of Ireland, and I sit in the editorial boards of many journals as well as the PhD examination boards of national and international universities. I have lead a team of enviroCORE researchers in a European collaborative consortium securing 3.7 million euro to work on nutrient conservation in soils. I work with the Directors of the other COREs of the Institute to develop common projects and I currently collaborate in a gameCORE initiated research project. Moreover, recently I have stated collaboration with the industry giant Alltech on the development of sustainable plant parasitic nematode approaches. I am an avid participant in Institute out-reach and promotion activities, contributing to the Eureka Carlow Festival, organising Science Week visits to  Teagasc Oak Park, judging in SciFest and carrying out school visits. I also promote enviroCORE and SETU Carlow to stakeholders, such as the Irish Farmers Association, the Carlow Enterprise Board, Carlow County Council and the Southern Region Waste Management Office, and others.